MASS SPECTROMETRY AND ITS PRINCIPLE
MASS SPECTROMETRY AND ITS PRINCIPLE Mass spectrometry is an analytical technique used to quantify material or identify unknown materials. the process involves the conversion of samples into a gaseous form which are then differentiated by their mass to charge ratio (m/ z). ions are separated on the availability and abundance in the mass spectrum. The mass spectrum shows the plot of ion abundance versus charge–to–mass ratio. Ion gives the information of the structure and nature of the prime molecule of the sample. The basic premise of mass spectrometry (MS) is to create ions from inorganic or organic substances using any acceptable method, separate them using their mass-to-charge ratio (m/z), and detect them qualitatively and quantitatively using their m/z and abundance. Thermally, by electric fields, or by striking energetic electrons, ions , or photons, the analyte can be ionized . Single ionized atoms, clusters, molecules, or their fragments or partners can all be considered ions. St